Evaluation of domestic animal sperm head morphology via flow cytometric DNA labelling and pulse shape analysis using bull and stallion spermatozoa as model species.
Reprod Domest Anim
; 58(11): 1569-1575, 2023 Nov.
Article
em En
| MEDLINE
| ID: mdl-37706243
ABSTRACT
The aim of the present study was to test a rapid, robust flow cytometric technique for the detection of sperm head abnormalities of domestic bulls and stallions. The so-called PulSA approach detects the pulse profiles of propidium-iodide labelled spermatozoa. In the first experiment, species-specific threshold values were established on sperm samples that were tested for sperm head abnormalities with a classic visual morphology analysis. In the second experiment, serial mixtures of bull and stallion spermatozoa mimicking different percentages of sperm head abnormalities were analysed. Non-metric multidimensional scaling showed a clear separation between the normal and mixed samples. The PulSA approach may be a useful tool in identifying sub- or infertile breeding males as well as in studying the evolutionary aspects of sperm morphology and morphometry.
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Base de dados:
MEDLINE
Assunto principal:
Doenças dos Bovinos
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Doenças dos Cavalos
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Infertilidade Masculina
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article