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Generation of a TRPV1 knockout human pluripotent stem cell line (WAe009-A-U) using CRISPR/Cas9.
Cao, Yuhong; Tang, Ling; Su, Jun; Wang, Hao; Liang, Ping; Rong, Kuang; Gong, Tingyu.
Afiliação
  • Cao Y; Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China; NMPA Key Laboratory for Animal Alternati
  • Tang L; Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China.
  • Su J; Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China.
  • Wang H; Prenatal Diagnosis Center, Hangzhou Women's Hospital, Hangzhou 310008, China.
  • Liang P; Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China. Electronic address: pingliang@zju.edu.cn
  • Rong K; NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetis, Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China. Electronic address: 287640065@qq.com.
  • Gong T; Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China. Electronic address: 11918392@zju
Stem Cell Res ; 72: 103202, 2023 10.
Article em En | MEDLINE | ID: mdl-37708613
ABSTRACT
The transient receptor potential vanilloid subfamily 1 (TRPV1) is a polymodal nociceptor that is highly expressed in sensory nerves. Activation of TRPV1 receptors excites primary afferent nociceptors by opening cation channels, allowing the influx of Na+ and Ca2+ ions into the cytoplasm. Here, a TRPV1 knockout human embryonic stem cell line was generated using the CRISPR/Cas9 genome-editing technology to further study the function of TRPV1. The cell line confirmed with normal pluripotency and karyotype.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Células-Tronco Embrionárias Humanas Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Células-Tronco Embrionárias Humanas Idioma: En Ano de publicação: 2023 Tipo de documento: Article