Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages.
STAR Protoc
; 4(4): 102559, 2023 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-37713309
ABSTRACT
Protein synthesis, or mRNA translation, is the biological process through which genetic information stored in messenger RNAs is encoded into proteins. Here, we present an optimized protocol for assessing the translation rate in mouse adult microglia and cultured bone-marrow-derived macrophages. We describe steps for isolating cells, treating them with a puromycin-analog probe, and fluorescently labeling the puromycylated-polypeptide chains. We then detail their quantification by flow cytometry or with a fluorescent plate reader. For complete details on the use and execution of this protocol, please refer to Keane et al. (2021).1.
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Base de dados:
MEDLINE
Assunto principal:
Medula Óssea
/
Microglia
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article