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Unveiling the Pharmacological Significance of Marine Streptomyces violaceusniger KS20: Isolation, Characterization, and Assessment of Its Biomedical Applications.
Chakraborty, Bidhayak; Shashiraj, Kariyellappa Nagaraja; Kumar, Raju Suresh; Bhat, Meghashyama Prabhakara; Basavarajappa, Dhanyakumara Shivapoojar; Almansour, Abdulrahman I; Perumal, Karthikeyan; Nayaka, Sreenivasa.
Afiliação
  • Chakraborty B; P.G. Department of Studies in Botany, Karnatak University, Dharwad 580003, India.
  • Shashiraj KN; P.G. Department of Studies in Botany, Karnatak University, Dharwad 580003, India.
  • Kumar RS; Department of Chemistry, College of Science, King Saud University, P.O. Box. 2455, Riyadh 11451, Saudi Arabia.
  • Bhat MP; P.G. Department of Studies in Botany, Karnatak University, Dharwad 580003, India.
  • Basavarajappa DS; P.G. Department of Studies in Botany, Karnatak University, Dharwad 580003, India.
  • Almansour AI; Department of Chemistry, College of Science, King Saud University, P.O. Box. 2455, Riyadh 11451, Saudi Arabia.
  • Perumal K; Department of Chemistry and Biochemistry, The Ohio State University, 151 W. Woodruff Ave, Columbus, OH 43210, USA.
  • Nayaka S; P.G. Department of Studies in Botany, Karnatak University, Dharwad 580003, India.
Metabolites ; 13(9)2023 Sep 19.
Article em En | MEDLINE | ID: mdl-37755302
Marine actinomycetes represent a highly favorable source of bioactive compounds and have been the mainstay of much research in recent years. Recent reports have shown that marine Streptomyces sp. can produce compounds with diverse and potent biological activities. Therefore, the key objective of the study was to isolate and screen a potential actinomycete from marine ecosystems of Devbagh and Tilmati beaches, Karwar. Streptomyces sp. KS20 was characterized and the ethyl acetate extract (EtOAc-Ex) was screened for biomedical applications. Streptomyces sp. KS20 produced grayish-white aerial and pale-yellow substrate mycelia and revealed an ancestral relationship with Streptomyces violaceusniger. Optimum growth of the organism was recorded at 30 °C and pH 7.0. The metabolite profiling of EtOAc-Ex expressed the existence of several bioactive metabolites, whereas the functional groups were indicated by Fourier transform infrared (FTIR) spectroscopy. A considerable antioxidant activity was shown for EtOAc-Ex with IC50 of 92.56 µg/mL. In addition to this, Streptomyces sp. KS20 exhibited significant antimicrobial properties, particularly against Escherichia coli, where a zone of inhibition measuring 36 ± 0.83 mm and a minimum inhibitory concentration (MIC) of 3.12 µg/mL were observed. The EtOAc-Ex even revealed significant antimycobacterial potency with IC50 of 6.25 µg/mL. Finally, the antiproliferative potentiality of EtOAc-Ex against A549 and PC-3 cell lines revealed a constant decline in cell viability while raising the concentration of EtOAc-Ex from 12.5 to 200 µg/mL. The IC50 values were determined as 94.73 µg/mL and 121.12 µg/mL for A549 and PC-3 cell lines, respectively. Overall, the exploration of secondary metabolites from marine Streptomyces sp. KS20 represents an exciting area of further research with the potential to discover novel bioactive compounds that could be developed into therapeutics for various medical applications.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article