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The multi-omics single-cell landscape of sinus mucosa in uncontrolled severe chronic rhinosinusitis with nasal polyps.
Xu, Zhaofeng; Huang, Yanran; Meese, Tim; Van Nevel, Sharon; Holtappels, Gabriele; Vanhee, Stijn; Bröker, Barbara M; Li, Zhengqi; de Meester, Ellen; De Ruyck, Natalie; Van Zele, Thibaut; Gevaert, Philip; Van Nieuwerburgh, Filip; Zhang, Luo; Shamji, Mohamed H; Wen, Weiping; Zhang, Nan; Bachert, Claus.
Afiliação
  • Xu Z; The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Department of Otorhinolaryngology, International Airway Research Center, Guangzhou, China; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium.
  • Huang Y; The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Department of Otorhinolaryngology, International Airway Research Center, Guangzhou, China; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium; Department of Allergy, Department of Otolaryngology Head and
  • Meese T; NXTGNT, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
  • Van Nevel S; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium.
  • Holtappels G; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium.
  • Vanhee S; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium; VIB-UGent, Center for Inflammation Research, Gent 9052, Belgium.
  • Bröker BM; Institute of Immunology, University Medicine Greifswald, Greifswald, Germany.
  • Li Z; The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Department of Otorhinolaryngology, International Airway Research Center, Guangzhou, China.
  • de Meester E; NXTGNT, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
  • De Ruyck N; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium.
  • Van Zele T; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium.
  • Gevaert P; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium.
  • Van Nieuwerburgh F; NXTGNT, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
  • Zhang L; Department of Allergy, Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, PR China; Beijing key laboratory of nasal diseases, Beijing Institute of Otolaryngology, Beijing, PR China.
  • Shamji MH; National Heart and Lung Institute, Imperial College London, and NIHR Imperial Biomedical Research Centre, United Kingdom.
  • Wen W; The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Department of Otorhinolaryngology, International Airway Research Center, Guangzhou, China; The Sixth Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, China. Electronic address: wenwp@mai
  • Zhang N; The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Department of Otorhinolaryngology, International Airway Research Center, Guangzhou, China; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium. Electronic address: nan.zhang@ugent.be.
  • Bachert C; The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Department of Otorhinolaryngology, International Airway Research Center, Guangzhou, China; Upper Airway Research Laboratory, Ghent University, Ghent, Belgium; Clinic for ENT diseases and head and neck surgery, Universit
Clin Immunol ; 256: 109791, 2023 11.
Article em En | MEDLINE | ID: mdl-37769787
Uncontrolled severe chronic rhinosinusitis with nasal polyps (CRSwNP) is associated with elevated levels of type 2 inflammatory cytokines and raised immunoglobulin concentrations in nasal polyp tissue. By using single-cell RNA sequencing, transcriptomics, surface proteomics, and T cell and B cell receptor sequencing, we found the predominant cell types in nasal polyps were shifted from epithelial and mesenchymal cells to inflammatory cells compared to nasal mucosa from healthy controls. Broad expansions of CD4 T effector memory cells, CD4 tissue-resident memory T cells, CD8 T effector memory cells and all subtypes of B cells in nasal polyp tissues. The T and B cell receptor repertoires were skewed in NP. This study highlights the deviated immune response and remodeling mechanisms that contribute to the pathogenesis of uncontrolled severe CRSwNP. CLINICAL IMPLICATIONS: We identified differences in the cellular compositions, transcriptomes, proteomes, and deviations in the immune profiles of T cell and B cell receptors as well as alterations in the intercellular communications in uncontrolled severe CRSwNP patients versus healthy controls, which might help to define potential therapeutic targets in the future.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sinusite / Rinite / Pólipos Nasais Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sinusite / Rinite / Pólipos Nasais Idioma: En Ano de publicação: 2023 Tipo de documento: Article