Efficient clathrin-mediated entry of enteric adenoviruses in human duodenal cells.
J Virol
; 97(10): e0077023, 2023 10 31.
Article
em En
| MEDLINE
| ID: mdl-37823645
ABSTRACT
IMPORTANCE Enteric adenoviruses have historically been difficult to grow in cell culture, which has resulted in lack of knowledge of host factors and pathways required for infection of these medically relevant viruses. Previous studies in non-intestinal cell lines showed slow infection kinetics and generated comparatively low virus yields compared to other adenovirus types. We suggest duodenum-derived HuTu80 cells as a superior cell line for studies to complement efforts using complex intestinal tissue models. We show that viral host cell factors required for virus entry differ between cell lines from distinct origins and demonstrate the importance of clathrin-mediated endocytosis.
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Base de dados:
MEDLINE
Assunto principal:
Adenoviridae
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Clatrina
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Endocitose
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Internalização do Vírus
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article