Efficient Genome Editing in Most <i>Staphylococcus aureus</i> by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit.

Yang, Ping; Yang, Junjie; Lin, Ting; Liu, Qi; Yin, Yu; Chen, Daijie; Yang, Sheng

Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit.

Yang, Ping; Yang, Junjie; Lin, Ting; Liu, Qi; Yin, Yu; Chen, Daijie; Yang, Sheng.

Afiliação

Yang P; School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Yang J; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China.
Lin T; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Liu Q; School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Yin Y; School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Chen D; School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Yang S; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China.

ACS Synth Biol ; 12(11): 3340-3351, 2023 11 17.

Article em En | MEDLINE | ID: mdl-37830328

Assuntos

Edição de Genes; Staphylococcus aureus; Humanos; Staphylococcus aureus/genética; Sistemas CRISPR-Cas/genética; Enzimas de Restrição-Modificação do DNA/genética; Plasmídeos/genética

Palavras-chave

CRISPR-Cas9; Staphylococcus aureus; genome editing; restriction-modification; synthetic DNA; transformation efficiency

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Edição de Genes Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo

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PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Edição de Genes Idioma: En Ano de publicação: 2023 Tipo de documento: Article