Cytoprotective effect of propolis on heat stress induces alteration to histological, ultrastructural, and oxidative stress in catfish (Clarias gariepinus).
Environ Sci Pollut Res Int
; 30(53): 114152-114165, 2023 Nov.
Article
em En
| MEDLINE
| ID: mdl-37855965
Our study helps to evaluate the immune response, antioxidative status, and resistance against heat stress (HS) in Clarias gariepinus treated with propolis extraction; the results will contribute to theories of fish physiology and immunity under high-temperature conditions. Forty-five fish were divided into three equal groups: the control, the HS group at 36 °C, and the HS treated with alcoholic extraction of propolis that dissolved in water for 3 weeks. The results of our study suggested that the stress response differs among tissues thymus, spleen, and liver. All the tissues showed alteration in morphological and cytological structure at the light microscope (LM) and transmission electron microscope (TEM); thymus showed edema and thymocyte destruction; the spleen detected collagen deposition, and the liver displayed endoplasmic reticulum amplification (ER). In addition, we examined oxidative stress and antioxidant defenses (lipid peroxidation, catalase, and glutathione) of the spleen and measured blood biochemical parameters (alanine transaminase and aspartic transaminase levels) after heat stress. However, this toxic effect of HS was neutralized by the propolis extraction. To conclude, propolis is recommended to cope with the impacts of heat stress on catfish (Clarias gariepinus) by improving immunity and antioxidative resistance.
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Base de dados:
MEDLINE
Assunto principal:
Própole
/
Peixes-Gato
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article