Advancements in 3D spheroid imaging: Optimised cryosectioning and immunostaining techniques.

Charlet-Faure, Claire; Thulesen, Annemette Præstegaard; Rogowska-Wrzesinska, Adelina

Charlet-Faure, Claire; Thulesen, Annemette Præstegaard; Rogowska-Wrzesinska, Adelina.

Afiliação

Charlet-Faure C; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M DK-5230, Denmark.
Thulesen AP; Faculté des Sciences Site St Charles Aix Marseille Université, 3 place Victor Hugo, Marseille 13331, France.
Rogowska-Wrzesinska A; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M DK-5230, Denmark.

MethodsX ; 11: 102415, 2023 Dec.

Article em En | MEDLINE | ID: mdl-37860046

Palavras-chave

Antigen retrieval; Antigen retrieval and cryosectioning of multicellular 3D spheroids; Cryosectioning; Fluorescent microscopy; Immunostaining; Organoids; Spheroids

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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article