Your browser doesn't support javascript.
loading
Tumor heterogeneity and tumor-microglia interactions in primary and recurrent IDH1-mutant gliomas.
Blanco-Carmona, Enrique; Narayanan, Ashwin; Hernandez, Inmaculada; Nieto, Juan C; Elosua-Bayes, Marc; Sun, Xueyuan; Schmidt, Claudia; Pamir, Necmettin; Özduman, Koray; Herold-Mende, Christel; Pagani, Francesca; Cominelli, Manuela; Taranda, Julian; Wick, Wolfgang; von Deimling, Andreas; Poliani, Pietro Luigi; Rehli, Michael; Schlesner, Matthias; Heyn, Holger; Turcan, Sevin.
Afiliação
  • Blanco-Carmona E; Hopp Children's Cancer Center (KiTZ), Heidelberg, Germany; Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany.
  • Narayanan A; Neurology Clinic and National Center for Tumor Diseases, Heidelberg University Hospital and Heidelberg University, Heidelberg, Germany.
  • Hernandez I; Next Generation Sequencing Core, Leibniz Institute for Immunotherapy, c/o University Hospital Regensburg, Regensburg, Germany; CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain.
  • Nieto JC; CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain.
  • Elosua-Bayes M; CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain.
  • Sun X; Neurology Clinic and National Center for Tumor Diseases, Heidelberg University Hospital and Heidelberg University, Heidelberg, Germany; DKTK CCU Neurooncology, DKFZ, Heidelberg, Germany.
  • Schmidt C; Core Facility Unit Light Microscopy, DKFZ, Heidelberg, Germany.
  • Pamir N; Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Neurosurgery, Istanbul, Turkey.
  • Özduman K; Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Neurosurgery, Istanbul, Turkey.
  • Herold-Mende C; Division of Experimental Neurosurgery, Department of Neurosurgery, Heidelberg University Hospital, Heidelberg, Germany.
  • Pagani F; Pathology Unit, Department of Molecular and Translational Medicine, University of Brescia Medical School, Brescia, Italy.
  • Cominelli M; Pathology Unit, Department of Molecular and Translational Medicine, University of Brescia Medical School, Brescia, Italy.
  • Taranda J; Neurology Clinic and National Center for Tumor Diseases, Heidelberg University Hospital and Heidelberg University, Heidelberg, Germany; DKTK CCU Neurooncology, DKFZ, Heidelberg, Germany.
  • Wick W; Neurology Clinic and National Center for Tumor Diseases, Heidelberg University Hospital and Heidelberg University, Heidelberg, Germany; DKTK CCU Neurooncology, DKFZ, Heidelberg, Germany.
  • von Deimling A; Department of Neuropathology, Heidelberg University Hospital, and DKTK CCU Neuropathology, DKFZ, Heidelberg, Germany.
  • Poliani PL; Pathology Unit, Department of Molecular and Translational Medicine, University of Brescia Medical School, Brescia, Italy.
  • Rehli M; Next Generation Sequencing Core, Leibniz Institute for Immunotherapy, c/o University Hospital Regensburg, Regensburg, Germany; Department of Internal Medicine III, University Hospital Regensburg, Regensburg, Germany.
  • Schlesner M; Biomedical Informatics, Data Mining and Data Analytics, Faculty for Applied Informatics, University of Augsburg, Augsburg, Germany.
  • Heyn H; CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain; Universitat Pompeu Fabra, Barcelona, Spain. Electronic address: holger.heyn@cnag.eu.
  • Turcan S; Neurology Clinic and National Center for Tumor Diseases, Heidelberg University Hospital and Heidelberg University, Heidelberg, Germany; DKTK CCU Neurooncology, DKFZ, Heidelberg, Germany. Electronic address: sevin.turcan@med.uni-heidelberg.de.
Cell Rep Med ; 4(11): 101249, 2023 11 21.
Article em En | MEDLINE | ID: mdl-37883975
The isocitrate dehydrogenase (IDH) gene is recurrently mutated in adult diffuse gliomas. IDH-mutant gliomas are categorized into oligodendrogliomas and astrocytomas, each with unique pathological features. Here, we use single-nucleus RNA and ATAC sequencing to compare the molecular heterogeneity of these glioma subtypes. In addition to astrocyte-like, oligodendrocyte progenitor-like, and cycling tumor subpopulations, a tumor population enriched for ribosomal genes and translation elongation factors is primarily present in oligodendrogliomas. Longitudinal analysis of astrocytomas indicates that the proportion of tumor subpopulations remains stable in recurrent tumors. Analysis of tumor-associated microglia/macrophages (TAMs) reveals significant differences between oligodendrogliomas, with astrocytomas harboring inflammatory TAMs expressing phosphorylated STAT1, as confirmed by immunohistochemistry. Furthermore, inferred receptor-ligand interactions between tumor subpopulations and TAMs may contribute to TAM state diversity. Overall, our study sheds light on distinct tumor populations, TAM heterogeneity, TAM-tumor interactions in IDH-mutant glioma subtypes, and the relative stability of tumor subpopulations in recurrent astrocytomas.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligodendroglioma / Astrocitoma / Neoplasias Encefálicas / Glioma Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligodendroglioma / Astrocitoma / Neoplasias Encefálicas / Glioma Idioma: En Ano de publicação: 2023 Tipo de documento: Article