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Assessing the effects of single and binary exposures of copper and lead on Mytilus galloprovincialis: Physiological and genotoxic approaches.
Crowther, Charlotte; Turner, Andrew; Moore, Michael N; Jha, Awadhesh N.
Afiliação
  • Crowther C; School of Biological and Marine Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK.
  • Turner A; School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: aturner@plymouth.ac.uk.
  • Moore MN; School of Biological and Marine Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK; Plymouth Marine Laboratory, Prospect Place, Plymouth PL1 3DH, UK; European Centre for Environment and Human Health (ECEHH), Knowledge Spa, Royal Cornwall Hospital, University of Exeter Medical Schoo
  • Jha AN; School of Biological and Marine Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK.
Aquat Toxicol ; 265: 106741, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37944325
ABSTRACT
It is becoming increasingly recognised that contaminants are not isolated in their threats to the aquatic environment, with recent shifts towards studying the effects of chemical mixtures. In this study, adult marine mussels (Mytilus galloprovincialis) were exposed to two aqueous concentrations of the essential trace metal, Cu (5 and 32 µg L-1), and the non-essential metal, Pb (5 and 25 µg L-1), both individually and in binary mixtures. After a 14-day exposure, metal accumulation was determined in the digestive gland, gill and mantle tissues by inductively coupled plasma-mass spectrometry following acid digestion, and a number of biochemical, neurotoxic and physiological markers were assessed. These included measurements of DNA damage using comet assay, total glutathione concentration, acetylcholinesterase (AChE) activity and clearance rate. Metal accumulation was greater in the digestive gland and gill than in the mantle, and based on computed free ion concentrations, was greater for Pb than for Cu. Copper exhibited an inhibitory effect on Pb accumulation but Pb did not appear to affect Cu accumulation. Comet assay results revealed DNA damage (i.e., genotoxic effects) in all treatments but differences between the exposures were not significant (p > 0.05), and there were no significant differences in AChE activities between treatments. The most distinctive impacts were a reduction in clearance rate resulting from the higher concentration of Cu, with and without Pb, and an increase in glutathione in the gill resulting from the higher concentration of Cu without Pb. Multivariate analysis facilitated the development of a conceptual model based on the current findings and previously published data on the toxicity and intracellular behaviour of Cu and Pb that will assist in the advancement of regulations and guidelines regarding multiple metal contaminants in the environment.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Mytilus Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Mytilus Idioma: En Ano de publicação: 2023 Tipo de documento: Article