Single-Cell PET Imaging and Tracking.
Methods Mol Biol
; 2729: 331-340, 2024.
Article
em En
| MEDLINE
| ID: mdl-38006505
ABSTRACT
Positron emission tomography (PET) is one of the most sensitive whole-body molecular imaging techniques available in the clinic, able to detect picomolar levels of probe. As such, it was recently demonstrated that PET could also be used to track single radiolabeled cells in small animals. In this protocol, we present detailed procedures for radiolabeling cells using mesoporous silica nanoparticles (MSNs) and for tracking these cells in real time using in vivo PET. This includes static imaging of single cells as well as dynamic tracking of moving cells directly from the list-mode data. The protocol provides detailed instructions and examples for each step.
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Base de dados:
MEDLINE
Assunto principal:
Tomografia por Emissão de Pósitrons
/
Nanopartículas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article