Ready-to-use nanopore platform for label-free small molecule quantification: Ethanolamine as first example.
Nanomedicine
; 55: 102724, 2024 Jan.
Article
em En
| MEDLINE
| ID: mdl-38007066
ABSTRACT
In recent decades, nanopores have become a promising diagnostic tool. Protein and solid-state nanopores are increasingly used for both RNA/DNA sequencing and small molecule detection. The latter is of great importance, as their detection is difficult or expensive using available methods such as HPLC or LC-MS. DNA aptamers are an excellent detection element for sensitive and specific detection of small molecules. Herein, a method for quantifying small molecules using a ready-to-use sequencing platform is described. Taking ethanolamine as an example, a strand displacement assay is developed in which the target-binding aptamer is displaced from the surface of magnetic particles by ethanolamine. Non-displaced aptamer and thus the ethanolamine concentration are detected by the nanopore system and can be quantified in the micromolar range using our in-house developed analysis software. This method is thus the first to describe a label-free approach for the detection of small molecules in a protein nanopore system.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Aptâmeros de Nucleotídeos
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Nanoporos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article