Coronin-1 promotes directional cell rearrangement in Drosophila wing epithelium.
Cell Struct Funct
; 48(2): 251-257, 2023 Dec 20.
Article
em En
| MEDLINE
| ID: mdl-38030242
ABSTRACT
Directional cell rearrangement is a critical process underlying correct tissue deformation during morphogenesis. Although the involvement of F-actin regulation in cell rearrangement has been established, the role and regulation of actin binding proteins (ABPs) in this process are not well understood. In this study, we investigated the function of Coronin-1, a WD-repeat actin-binding protein, in controlling directional cell rearrangement in the Drosophila pupal wing. Transgenic flies expressing Coronin-1-EGFP were generated using CRISPR-Cas9. We observed that Coronin-1 localizes at the reconnecting junction during cell rearrangement, which is dependent on actin interacting protein 1 (AIP1) and cofilin, actin disassemblers and known regulators of wing cell rearrangement. Loss of Coronin-1 function reduces cell rearrangement directionality and hexagonal cell fraction. These results suggest that Coronin-1 promotes directional cell rearrangement via its interaction with AIP1 and cofilin, highlighting the role of ABPs in the complex process of morphogenesis.Key words morphogenesis, cell rearrangement, actin binding proteins (ABPs).
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MEDLINE
Assunto principal:
Drosophila
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Proteínas dos Microfilamentos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article