Your browser doesn't support javascript.
loading
The Combination of Mechanically Isolated Stromal Vascular Fraction and Fibrin Hydrogel: A Processing Protocol.
Pfister, Pablo; Reid, Gregory; Breckwoldt, Tabea; Vasella, Mauro; Seitz, Ann-Kathrin; Song, Seung Yong; Kim, Bong-Sung.
Afiliação
  • Pfister P; Department of Surgery, Triemli City Hospital Zurich.
  • Reid G; Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich.
  • Breckwoldt T; Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich.
  • Vasella M; Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich.
  • Seitz AK; Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich.
  • Song SY; Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine.
  • Kim BS; Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich; bong-sung.kim@usz.ch.
J Vis Exp ; (201)2023 Nov 17.
Article em En | MEDLINE | ID: mdl-38047553
The regenerative potential of adipose-derived stromal cells (ASCs) has gained significant attention in regenerative and translational research. In the past, the extraction of these cells from adipose tissue required a multistep enzyme-based process, resulting in a heterogenous cell mix consisting of ACSs and other cells, which are jointly termed the stromal vascular fraction (SVF). More recently introduced mechanical SVF (mSVF) isolation protocols are less time-consuming and bypass regulatory concerns. We recently proposed a protocol that generates mSVF rich in stromal cells based on a combination of emulsification and centrifugation. One current issue in mSVF application for wound therapy application is the lack of a scaffold providing protection from mechanical manipulation and desiccation. Fibrin hydrogels have been shown to be a useful adjunct in cell transfer for wound healing purposes in the past. In the work herein, we delineate the preparation steps of an mSVF-fibrin hydrogel construct as a novel approach for translational research and clinical application.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fibrina / Fração Vascular Estromal Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fibrina / Fração Vascular Estromal Idioma: En Ano de publicação: 2023 Tipo de documento: Article