Sustainable asymmetric synthesis of diltiazem precursor enabled by recombinant Escherichia coli whole cells co-expressing an engineered ketoreductase and glucose dehydrogenase.

Yue, Xiaoping; Li, Yitong; Yang, Lin; Sang, Di; Huang, Zedu; Chen, Fener

Yue, Xiaoping; Li, Yitong; Yang, Lin; Sang, Di; Huang, Zedu; Chen, Fener.

Afiliação

Yue X; Engineering Center of Catalysis and Synthesis for Chiral Molecules, Department of Chemistry, Fudan University, Shanghai, P. R. China.
Li Y; Shanghai Engineering Research Center of Industrial Asymmetric Catalysis of Chiral Drugs, Shanghai, P. R. China.
Yang L; Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu, P. R. China.
Sang D; Engineering Center of Catalysis and Synthesis for Chiral Molecules, Department of Chemistry, Fudan University, Shanghai, P. R. China.
Huang Z; Shanghai Engineering Research Center of Industrial Asymmetric Catalysis of Chiral Drugs, Shanghai, P. R. China.
Chen F; Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu, P. R. China.

Biotechnol J ; 19(1): e2300250, 2024 Jan.

Article em En | MEDLINE | ID: mdl-38048389

Assuntos

Diltiazem; Glucose 1-Desidrogenase; Glucose 1-Desidrogenase/metabolismo; Diltiazem/metabolismo; Escherichia coli/metabolismo; Propionatos/metabolismo; Biocatálise

Palavras-chave

(3S)-methyl 2-chloro-3-hydroxy-3-(4-methoxyphenyl)propanoate; co-expression; diltiazem; ketoreductase; semi-rational protein engineering

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Diltiazem / Glucose 1-Desidrogenase Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Diltiazem / Glucose 1-Desidrogenase Idioma: En Ano de publicação: 2024 Tipo de documento: Article