In Situ Stability Test for mRNA Vaccines Based on Deep-UV Resonance Raman Spectroscopy.
Anal Chem
; 96(3): 1003-1008, 2024 01 23.
Article
em En
| MEDLINE
| ID: mdl-38052070
ABSTRACT
Deep-UV resonance Raman spectroscopy has been shown to offer great potential for probing the in situ stability of mRNA vaccines. In this study, a vaccine model was subjected to controlled degradation using RNase A or through aging at room temperature. The degradation of mRNA was confirmed by using a cell transfection test and by gel electrophoresis. Under both settings, DUVRR spectroscopy successfully revealed the mRNA degradation signs of the vaccine model.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
/
Vacinas de mRNA
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article