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Enzyme-free electrochemical biosensor based on bio-barcode amplification for ultra-sensitive detection of microRNA.
Xi, Huai; Liang, Xiaolin; Huang, Guidan; Liang, Jinhua; Li, Dandan; Wen, Qilin; Zhang, Yun; Xiao, Xiaofen; Zhu, Wenyuan.
Afiliação
  • Xi H; College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
  • Liang X; College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
  • Huang G; College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
  • Liang J; College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
  • Li D; College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
  • Wen Q; College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
  • Zhang Y; College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
  • Xiao X; Key Laboratory of Carbon Materials of Zhejiang Province, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, 325035, Zhejiang, China. xiaoxf3@mail2.sysu.edu.cn.
  • Zhu W; College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China. wyzhu@glut.edu.cn.
Anal Sci ; 40(2): 285-290, 2024 Feb.
Article em En | MEDLINE | ID: mdl-38062249
ABSTRACT
The rapid and accurate detection of miRNAs is of great significance for early diagnosis and treatment of cancer. Hence, a novel enzyme-free and label-free electrochemical biosensor based on bio-barcode amplification for detecting miRNAs was presented. Sandwich structures constructed of magnetic nanoparticles modified with DNA probes, gold nanoparticles with numerous barcoded DNA strands that hybridized with target miRNAs were fabricated as the amplifier. The released barcoded DNA strands then acted as the secondary targets and triggered the electrochemical sensor with a significant electrochemical response. A highly sensitive (detection limit of 0.24 fM) and selective electrochemical miRNA detection was realized, which has great potential for application in miRNA-related clinical diagnosis and biochemical research.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / MicroRNAs / Nanopartículas Metálicas Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / MicroRNAs / Nanopartículas Metálicas Idioma: En Ano de publicação: 2024 Tipo de documento: Article