Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA.
Methods Mol Biol
; 2733: 87-99, 2024.
Article
em En
| MEDLINE
| ID: mdl-38064028
ABSTRACT
The piscine orthomyxovirus called infectious salmon anemia virus (ISAV) is one of the most important emerging pathogens affecting the salmon industry worldwide. The first reverse genetics system for ISAV, which allows the generation of recombinant ISA virus (rISAV), is an important tool for the characterization and study of this virus. The plasmid-based reverse genetics system for ISAV includes the use of a novel fish promoter, the Atlantic salmon internal transcribed spacer region 1 (ITS-1). The salmon, viral, and mammalian genetic elements included in the pSS-URG vectors allow the expression of the eight viral RNA segments. In addition to four cytomegalovirus (CMV)-based vectors that express the four proteins of the ISAV ribonucleoprotein complex, the eight pSS-URG vectors allowed the generation of infectious rISAV in salmon cells.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Orthomyxoviridae
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Infecções por Orthomyxoviridae
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Isavirus
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Doenças dos Peixes
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article