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Transcriptomic and metabolomic analyses reveals keys genes and metabolic pathways in tea (Camellia sinensis) against six-spotted spider mite (Eotetranychus Sexmaculatus).
Wang, Xiaoping; Xiang, Yunjia; Sun, Minshan; Xiong, Yuanyuan; Li, Chunhua; Zhang, Ting; Ma, Weiwei; Wang, Yun; Liu, Xiao.
Afiliação
  • Wang X; Tea Refining and Innovation Key Laboratory of Sichuan Province, Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China. wangxp922@sina.com.
  • Xiang Y; Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, China.
  • Sun M; Henan Assist Research Biotechnology Co., Ltd, Zhengzhou, China.
  • Xiong Y; Tea Refining and Innovation Key Laboratory of Sichuan Province, Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
  • Li C; Tea Refining and Innovation Key Laboratory of Sichuan Province, Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
  • Zhang T; Tea Refining and Innovation Key Laboratory of Sichuan Province, Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
  • Ma W; Tea Refining and Innovation Key Laboratory of Sichuan Province, Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
  • Wang Y; Tea Refining and Innovation Key Laboratory of Sichuan Province, Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
  • Liu X; Tea Refining and Innovation Key Laboratory of Sichuan Province, Tea Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
BMC Plant Biol ; 23(1): 638, 2023 Dec 11.
Article em En | MEDLINE | ID: mdl-38072959
BACKGROUND: Six-spotted spider mite (Eotetranychus sexmaculatus) is one of the most damaging pests of tea (Camellia sinensis). E. sexmaculatus causes great economic loss and affects tea quality adversely. In response to pests, such as spider mites, tea plants have evolved resistance mechanisms, such as expression of defense-related genes and defense-related metabolites. RESULTS: To evaluate the biochemical and molecular mechanisms of resistance in C. sinensis against spider mites, "Tianfu-5" (resistant to E. sexmaculatus) and "Fuding Dabai" (susceptible to E. sexmaculatus) were inoculated with spider mites. Transcriptomics and metabolomics based on RNA-Seq and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) technology were used to analyze changes in gene expression and metabolite content, respectively. RNA-Seq data analysis revealed that 246 to 3,986 differentially expressed genes (DEGs) were identified in multiple compared groups, and these DEGs were significantly enriched in various pathways, such as phenylpropanoid and flavonoid biosynthesis, plant-pathogen interactions, MAPK signaling, and plant hormone signaling. Additionally, the metabolome data detected 2,220 metabolites, with 194 to 260 differentially abundant metabolites (DAMs) identified in multiple compared groups, including phenylalanine, lignin, salicylic acid, and jasmonic acid. The combined analysis of RNA-Seq and metabolomic data indicated that phenylpropanoid and flavonoid biosynthesis, MAPK signaling, and Ca2+-mediated PR-1 signaling pathways may contribute to spider mite resistance. CONCLUSIONS: Our findings provide insights for identifying insect-induced genes and metabolites and form a basis for studies on mechanisms of host defense against spider mites in C. sinensis. The candidate genes and metabolites identified will be a valuable resource for tea breeding in response to biotic stress.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Camellia sinensis / Tetranychidae Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Camellia sinensis / Tetranychidae Idioma: En Ano de publicação: 2023 Tipo de documento: Article