Macromolecular Crowding and DNA: Bridging the Gap between In Vitro and In Vivo.
Int J Mol Sci
; 24(24)2023 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-38139331
ABSTRACT
The cellular environment is highly crowded, with up to 40% of the volume fraction of the cell occupied by various macromolecules. Most laboratory experiments take place in dilute buffer solutions; by adding various synthetic or organic macromolecules, researchers have begun to bridge the gap between in vitro and in vivo measurements. This is a review of the reported effects of macromolecular crowding on the compaction and extension of DNA, the effect of macromolecular crowding on DNA kinetics, and protein-DNA interactions. Theoretical models related to macromolecular crowding and DNA are briefly reviewed. Gaps in the literature, including the use of biologically relevant crowders, simultaneous use of multi-sized crowders, empirical connections between macromolecular crowding and liquid-liquid phase separation of nucleic materials are discussed.
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Base de dados:
MEDLINE
Assunto principal:
DNA
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article