Yield stress analysis of cellulose nanocrystals (CNCs) in hyaluronic acid suspensions.

ABSTRACT

Due to their biodegradability features, cellulose nanocrystals (CNCs) and hyaluronic acid (HA) have been simultaneously used in the matrix of hydrogels for biomedical applications, such as corneal transplantation, and skin regeneration. Although rheology of these hydrogels may provide useful information for their applications, little to no attention has been paid to rheological characterization. In this study, we analyzed the rheology of HA-CNC suspensions and more specifically their yielding behavior. Through different rheological experiments, known as stress ramp, shear rate ramp and amplitude sweep; it was observed that HA-CNC gels possessed two yield points. Reproducible magnitudes of yield stress were obtained by optimizing the experimental conditions. The rheo-optics characterizations confirmed that the first and second yield points could be attributed to the bond and cage breakage phenomena. Studying the effect of concentration, the second yield stress increased linearly by CNC concentration, whereas the first yield point manifested a power-law dependence on concentration (exponent of 0.5). This power-law relationship was further justified by the evolution of average distance between the CNC individual particles (d), calculated through SAXS analysis.