Beyond fluorescence: advances in computational label-free full specificity in 3D quantitative phase microscopy.
Curr Opin Biotechnol
; 85: 103054, 2024 02.
Article
em En
| MEDLINE
| ID: mdl-38142647
ABSTRACT
Despite remarkable progresses in quantitative phase imaging (QPI) microscopes, their wide acceptance is limited due to the lack of specificity compared with the well-established fluorescence microscopy. In fact, the absence of fluorescent tag prevents to identify subcellular structures in single cells, making challenging the interpretation of label-free 2D and 3D phase-contrast data. Great effort has been made by many groups worldwide to address and overcome such limitation. Different computational methods have been proposed and many more are currently under investigation to achieve label-free microscopic imaging at single-cell level to recognize and quantify different subcellular compartments. This route promises to bridge the gap between QPI and FM for real-world applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imageamento Quantitativo de Fase
/
Microscopia
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article