Development of fluorescence-linked immunosorbent assay for rapid detection of Staphylococcus aureus.
Appl Microbiol Biotechnol
; 108(1): 2, 2024 Dec.
Article
em En
| MEDLINE
| ID: mdl-38153552
ABSTRACT
Staphylococcus aureus is a major pathogen that causes infections and life-threatening diseases. Although antibiotics, such as methicillin, have been used, methicillin-resistant S. aureus (MRSA) causes high morbidity and mortality rates, and conventional detection methods are difficult to be used because of time-consuming process. To control the spread of S. aureus, a development of a rapid and simple detection method is required. In this study, we generated a fluorescent anti-S. aureus antibody, and established a novel fluorescence-linked immunosorbent assay (FLISA)-based S. aureus detection method. The method showed high sensitivity and low limit of detection toward MRSA detection. The assay time for FLISA was 5 h, which was faster than that of conventional enzyme-linked immunosorbent assay (ELISA) or rapid ELISA. Moreover, the FLISA-based detection method was applied to diagnose clinically isolated MRSA samples that required only 5.3 h of preincubation. The FLISA method developed in this study can be widely applied as a useful tool for convenient S. aureus detection. KEY POINTS ⢠A fluorescence-linked immunosorbent assay-based S. aureus detection method ⢠Simultaneous quantification of a maximum of 96 samples within 5 h ⢠Application of the novel system to diagnosis clinical isolates.
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Base de dados:
MEDLINE
Assunto principal:
Infecções Estafilocócicas
/
Staphylococcus aureus Resistente à Meticilina
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article