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Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex.
Meka, Durga Praveen; Richter, Melanie; Rücker, Tabitha; Voss, Hannah; Rissiek, Anne; Krisp, Christoph; Kumar, Nisha Hemandhar; Schwanke, Birgit; Fornasiero, Eugenio F; Schlüter, Hartmut; Calderon de Anda, Froylan.
Afiliação
  • Meka DP; RG Neuronal Development, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
  • Richter M; RG Neuronal Development, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
  • Rücker T; RG Neuronal Development, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany. Electronic address: tabitha.m.ruecker@gmail.com.
  • Voss H; Institute for Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics Group, Campus Forschung, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
  • Rissiek A; Cytometry und Cell Sorting Core Unit, Department of Stem Cell Transplantation, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
  • Krisp C; Institute for Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics Group, Campus Forschung, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
  • Kumar NH; Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073 Göttingen, Germany.
  • Schwanke B; RG Neuronal Development, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
  • Fornasiero EF; Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073 Göttingen, Germany; Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
  • Schlüter H; Diagnostic Center, Section Mass Spectrometric Proteomics Group, Campus Forschung, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. Electronic address: hschluet@uke.de.
  • Calderon de Anda F; RG Neuronal Development, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany. Electronic address: froylan.calderon@zmnh.uni-hamburg.de.
STAR Protoc ; 5(1): 102793, 2024 Mar 15.
Article em En | MEDLINE | ID: mdl-38157295
ABSTRACT
Here, we present a protocol for differential multi-omic analyses of distinct cell types in the developing mouse cerebral cortex. We describe steps for in utero electroporation, subsequent flow-cytometry-based isolation of developing mouse cortical cells, bulk RNA sequencing or quantitative liquid chromatography-tandem mass spectrometry, and bioinformatic analyses. This protocol can be applied to compare the proteomes and transcriptomes of developing mouse cortical cell populations after various manipulations (e.g., epigenetic). For complete details on the use and execution of this protocol, please refer to Meka et al. (2022).1.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biologia Computacional / Multiômica Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biologia Computacional / Multiômica Idioma: En Ano de publicação: 2024 Tipo de documento: Article