LC-MS/MS method for simultaneous estimation of raloxifene, cladrin in rat plasma: application in pharmacokinetic studies.
Bioanalysis
; 16(3): 141-153, 2024 Feb.
Article
em En
| MEDLINE
| ID: mdl-38197392
ABSTRACT
Aim:
A newer LC-MS/MS method was developed and validated for the simultaneous quantification of raloxifene (RL) and cladrin (CL).Methodology:
Both drugs were resolved in RP-18 (4.6 × 50 mm, 5 µ) Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (7030% v/v) as mobile phase by using biological matrices in female Sprague-Dawley rats using-MS/MS.Results:
The developed method was found to be linear over the concentration ranges of 1-600 ng/ml, and lower limit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showed Cmax = 4.23 ± 0.61, 26.97 ± 1.14 ng/ml, at Tmax(h) 5.5 ± 1.00 and 3.5 ± 1.00, respectively.Conclusion:
Pharmacokinetic study results will be useful in the future for the combined delivery of RL and CL for osteoporosis treatment.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas em Tandem
/
Espectrometria de Massa com Cromatografia Líquida
/
Isoflavonas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article