A dual-mode fluorescence and colorimetric sensing platform for efficient detection of ofloxacin in aquatic products using iron alkoxide nanozyme.
Food Chem
; 442: 138417, 2024 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-38237297
ABSTRACT
Trace detection of ofloxacin (OFL) with high sensitivity, reliability, and visual clarity is challenging. To address this, a novel dual-modal aptasensor with fluorescence-colorimetric capabilities was designed that exploit the target-induced release of 3,3',5,5'-tetramethylbenzidine (TMB) molecules from aptamer-gated mesoporous silica nanoparticles (MSNs), the oxidase-like activity of iron alkoxide (IA) nanozyme, and the fluorescence attributes of core-shell upconversion nanoparticles. Therefore, the study reports a dual mode detection, with a fluorescence detection range for OFL spanning from 0.1 µg/kg to 1000 µg/kg (and a detection limit of 0.048 µg/kg). Additionally, the colorimetric method offered a linear detection range of 0.3 µg/kg to 1000 µg/kg, with a detection limit of 0.165 µg/kg. The proposed biosensor had been successfully applied to the determination of OFL content in real samples with satisfactory recoveries (78.24-96.14 %).
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MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Colorimetria
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article