Haplotype-resolved assembly of a tetraploid potato genome using long reads and low-depth offspring data.
Genome Biol
; 25(1): 26, 2024 01 19.
Article
em En
| MEDLINE
| ID: mdl-38243222
ABSTRACT
Potato is one of the world's major staple crops, and like many important crop plants, it has a polyploid genome. Polyploid haplotype assembly poses a major computational challenge. We introduce a novel strategy for the assembly of polyploid genomes and present an assembly of the autotetraploid potato cultivar Altus. Our method uses low-depth sequencing data from an offspring population to achieve chromosomal clustering and haplotype phasing on the assembly graph. Our approach generates high-quality assemblies of individual chromosomes with haplotype-specific sequence resolution of whole chromosome arms and can be applied in common breeding scenarios where collections of offspring are available.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Solanum tuberosum
/
Tetraploidia
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article