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Identification of Autoantigens in Pediatric Gastric Juices.
Youn, Hee-Shang; Jun, Jin-Su; Yeom, Jung Sook; Park, Ji Sook; Lim, Jae-Young; Woo, Hyang-Ok; Yang, Jung-Wook; Baik, Seung-Chul; Lee, Woo-Kon; Seo, Ji-Hyun.
Afiliação
  • Youn HS; Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Jun JS; Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Yeom JS; Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Park JS; Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Lim JY; Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Woo HO; Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Yang JW; Department of Pathology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Baik SC; Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Lee WK; Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea.
  • Seo JH; Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital, Gyeongsang Institute of Health Science, Jinju, Korea.
Pediatr Gastroenterol Hepatol Nutr ; 27(1): 15-25, 2024 Jan.
Article em En | MEDLINE | ID: mdl-38249638
ABSTRACT

Purpose:

This study aimed to investigate the presence of autoantigens in the gastric juices of children.

Methods:

Gastric juice and serum samples were obtained from 53 children <15 years of age who underwent gastric endoscopy. Among these, 8, 22, and 23 participants were in the age groups 0-5, 6-10, and 11-15 years, respectively. These samples were analyzed using two-dimensional electrophoresis (2-DE), immunoblot analysis, and matrix-assisted laser desorption ionization-time of-flight mass spectrometry. Furthermore, we reviewed the histopathological findings and urease test results and compared them with the results of 2-DE and immunoblot analysis.

Results:

There were no statistically significant differences in urease test positivity, grades of chronic gastritis, active gastritis, or Helicobacter pylori infiltration of the antrum and body among the three age groups. Three distinct patterns of gastric juice were observed on 2-DE. Pattern I was the most common, and pattern III was not observed below the age of 5 years. Histopathological findings were significantly different among active gastritis (p=0.037) and H. pylori infiltration (p=0.060) in the gastric body. The immunoblots showed large spots at an approximate pH of 3-4 and molecular weights of 31-45 kDa. These distinct, large positive spots were identified as gastric lipase and pepsin A and C.

Conclusion:

Three enzymes, which are normally secreted under acidic conditions were identified as autoantigens. Further investigation of the pathophysiology and function of autoantigens in the stomach is required.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article