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An improved pathway for autonomous bioluminescence imaging in eukaryotes.
Shakhova, Ekaterina S; Karataeva, Tatiana A; Markina, Nadezhda M; Mitiouchkina, Tatiana; Palkina, Kseniia A; Perfilov, Maxim M; Wood, Monika G; Hoang, Trish T; Hall, Mary P; Fakhranurova, Liliia I; Alekberova, Anna E; Malyshevskaia, Alena K; Gorbachev, Dmitry A; Bugaeva, Evgenia N; Pletneva, Ludmila K; Babenko, Vladislav V; Boldyreva, Daria I; Gorokhovatsky, Andrey Y; Balakireva, Anastasia V; Gao, Feng; Choob, Vladimir V; Encell, Lance P; Wood, Keith V; Yampolsky, Ilia V; Sarkisyan, Karen S; Mishin, Alexander S.
Afiliação
  • Shakhova ES; Planta LLC, Moscow, Russia.
  • Karataeva TA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Markina NM; Planta LLC, Moscow, Russia.
  • Mitiouchkina T; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Palkina KA; Planta LLC, Moscow, Russia.
  • Perfilov MM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Wood MG; Planta LLC, Moscow, Russia.
  • Hoang TT; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Hall MP; Planta LLC, Moscow, Russia.
  • Fakhranurova LI; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Alekberova AE; Planta LLC, Moscow, Russia.
  • Malyshevskaia AK; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Gorbachev DA; Promega Corporation, Madison, WI, USA.
  • Bugaeva EN; Promega Corporation, Madison, WI, USA.
  • Pletneva LK; Promega Corporation, Madison, WI, USA.
  • Babenko VV; Planta LLC, Moscow, Russia.
  • Boldyreva DI; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Gorokhovatsky AY; Planta LLC, Moscow, Russia.
  • Balakireva AV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Gao F; Planta LLC, Moscow, Russia.
  • Choob VV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • Encell LP; Planta LLC, Moscow, Russia.
  • Wood KV; Planta LLC, Moscow, Russia.
  • Yampolsky IV; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.
  • Sarkisyan KS; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.
  • Mishin AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Nat Methods ; 21(3): 406-410, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38253843
ABSTRACT
The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Luminescência / Eucariotos Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Luminescência / Eucariotos Idioma: En Ano de publicação: 2024 Tipo de documento: Article