A Novel Method for Real-Time Quantification of Radioligand Binding to Living Tumor Cells In Vitro.
Cancer Biother Radiopharm
; 39(1): 75-81, 2024 Feb.
Article
em En
| MEDLINE
| ID: mdl-38265676
ABSTRACT
Background:
Real-time quantification of radioligand binding to cells under in vivo-like conditions improves evaluation of clinical potential. Materials andMethods:
SKOV-3 tumor cells were grown in a monolayer on a thin glass plate placed in a sealable shallow chamber with a continuous flow of 125I-trastuzumab solution. The time-dependent cell binding was measured using a NaI detector, and the binding parameters were derived by computational analysis.Results:
The detection efficiency of 125I was 65 cps/kBq for radioligand bound to the cells. Experiments were analyzed to find the values of kon and koff. The resulting kon was 3.2-7.9 × 104 M-1 s-1 and koff was 0.11-4.2 × 10-5 s-1.Conclusions:
Radioligands can be rapidly evaluated by binding to living cells for selection and optimization of radioconjugates for diagnostic and therapeutic purposes.
Texto completo:
1
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article