Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System.
Methods Mol Biol
; 2766: 63-81, 2024.
Article
em En
| MEDLINE
| ID: mdl-38270868
ABSTRACT
Antibodies specifically recognizing integral membrane proteins are essential tools for functional analysis, diagnosis, and therapeutics targeting membrane proteins. However, developing antibodies against membrane proteins remains a big challenge because mass production of membrane proteins is difficult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using a cell-free protein synthesis method with liposome and dialysis cup. Here, we introduce practical and efficient integrated procedures to produce a large amount of proteoliposome antigen for anti-membrane protein antibody development.
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Base de dados:
MEDLINE
Assunto principal:
Proteolipídeos
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Diálise Renal
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Anticorpos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article