Rapid authentication of characteristic milk powders by recombinase polymerase amplification assays.
Food Chem
; 443: 138540, 2024 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-38277935
ABSTRACT
The authentication of dairy species has great significance for food safety. This study focused on a more rapid method for identifying major dairy species, and specific recombinase polymerase amplification (RPA)-based assays for cattle, goat, sheep, camel and donkey were developed. Through the developed RPA-based assays, goats and sheep could be simultaneously identified and bovine families could be differentiated. The performances of the RPA assays were validated using 37 milk powder samples, of which 16.2% (6/37) were suspected of being adulterated and 24.3% (9/37) were potentially at risk of being wrongly identified as adulteration. The effectiveness of the developed assays for crude DNA detection was also validated by a rapid nucleic acid extraction kit, and results showed that the presence of large amounts of protein and fat did not affect the qualitative results. Therefore, these assays could combine with the rapid nucleic acids extraction methods for being used in field detection.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos
/
Recombinases
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article