Determination of acetochlor by UPLC-MS3 in cells and its application to a cellular pharmacokinetic study.
Anal Biochem
; 688: 115476, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38286351
ABSTRACT
The aim of this work was to develop a fast, simple, and reliable UPLC-MS3 method for the sensitive detection of acetochlor in biological samples. In MS3 mode, the ion transition m/z 270.1 â 224.1â148.1 was chosen for quantification with butachlor as the internal standard. In the UPLC system, separation was performed on a UPLC column (2.1 × 50 mm ID, 1.7 µm) with 0.1 % FA in water and acetonitrile as mobile phases. After simple protein precipitation via acetonitrile, the method was well validated with good linearity (0.5-20 ng/mL, r > 0.995), accuracy (-3.70 %-2.98 %), and precision (<15 %). The selectivity and sensitivity were improved obviously in MS3 mode than that in MRM mode. The developed UPLC-MS3 method was successfully applied to the cellular pharmacokinetics study of acetochlor in MCF-7 cells.
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Base de dados:
MEDLINE
Assunto principal:
Toluidinas
/
Espectrometria de Massas em Tandem
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Espectrometria de Massa com Cromatografia Líquida
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article