Mass spectrometry imaging methods for visualizing tumor heterogeneity.
Curr Opin Biotechnol
; 86: 103068, 2024 04.
Article
em En
| MEDLINE
| ID: mdl-38310648
ABSTRACT
Profiling spatial distributions of lipids, metabolites, and proteins in tumors can reveal unique cellular microenvironments and provide molecular evidence for cancer cell dysfunction and proliferation. Mass spectrometry imaging (MSI) is a label-free technique that can be used to map biomolecules in tumors in situ. Here, we discuss current progress in applying MSI to uncover molecular heterogeneity in tumors. First, the analytical strategies to profile small molecules and proteins are outlined, and current methods for multimodal imaging to maximize biological information are highlighted. Second, we present and summarize biological insights obtained by MSI of tumor tissue. Finally, we discuss important considerations for designing MSI experiments and several current analytical challenges.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article