Protocol for neurogenin-2-mediated induction of human stem cell-derived neural progenitor cells.
STAR Protoc
; 5(1): 102878, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38335091
ABSTRACT
Human pluripotent stem cell-derived neural progenitor cells (NPCs) are an essential tool for the study of brain development and developmental disorders such as autism. Here, we present a protocol to generate NPCs rapidly and reproducibly from human stem cells using dual-SMAD inhibition coupled with a brief pulse of mouse neurogenin-2 (Ngn2) overexpression. We detail the 48-h induction scheme deployed to produce these cells-termed stem cell-derived Ngn2-accelerated progenitor cells-followed by steps for expansion, purification, banking, and quality assessment. For complete details on the use and execution of this protocol, please refer to Wells et al.1.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes
/
Células-Tronco Neurais
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article