Your browser doesn't support javascript.
loading
Effect of polystyrene nanoplastics exposure on gene expression and pathogenesis of zoonotic pathogen, Edwardsiella piscicida.
Yoon, Ju Bin; Yoon, Yongjoon; Park, Jeong Woo; Yerke, Aaron M; Jaiswal, Yogini S; Williams, Leonard L; Hwang, Sungmin; Moon, Ki Hwan.
Afiliação
  • Yoon JB; Laboratory of Marine Microbiology, Division of Convergence on Marine Science, National Korea Maritime and Ocean University, Busan 49112, South Korea; Department of Convergence Study on the Ocean Science and Technology, Ocean Science and Technology School, National Korea Maritime and Ocean University
  • Yoon Y; Laboratory of Marine Microbiology, Division of Convergence on Marine Science, National Korea Maritime and Ocean University, Busan 49112, South Korea; Department of Marine Bioscience and Environment, National Korea Maritime and Ocean University, Busan 49112, South Korea.
  • Park JW; Laboratory of Marine Microbiology, Division of Convergence on Marine Science, National Korea Maritime and Ocean University, Busan 49112, South Korea; Department of Convergence Study on the Ocean Science and Technology, Ocean Science and Technology School, National Korea Maritime and Ocean University
  • Yerke AM; Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
  • Jaiswal YS; Center for Excellence in Post Harvest Technologies, North Carolina Agricultural and Technical State University, The North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA.
  • Williams LL; Center for Excellence in Post Harvest Technologies, North Carolina Agricultural and Technical State University, The North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA.
  • Hwang S; Division of Practical Research, Honam National Institute Biological Resources, Mokpo-si 58762, South Korea. Electronic address: sh492@hnibr.re.kr.
  • Moon KH; Laboratory of Marine Microbiology, Division of Convergence on Marine Science, National Korea Maritime and Ocean University, Busan 49112, South Korea; Department of Convergence Study on the Ocean Science and Technology, Ocean Science and Technology School, National Korea Maritime and Ocean University
Ecotoxicol Environ Saf ; 272: 116057, 2024 Mar 01.
Article em En | MEDLINE | ID: mdl-38335574
ABSTRACT
A surge in the number of anthropogenic pollutants has been caused by increasing industrial activities. Nanoplastics are spotlighted as a new aquatic pollutant that are a threat to microbes and larger organisms. Our previous study showed that the subinhibitory concentrations of aquatic pollutants such as phenol and formalin act as signaling molecules and modulate global gene expression and metabolism. In this study, we aimed to investigate the impact of a new type of anthropogenic contaminant, polystyrene (PS) nanoplastics, on the expression of key virulence factors in zoonotic pathogen Edwardsiella piscicida and the assessment of potential changes in the susceptibility of zebrafish as a model host. The TEM data indicated a noticeable change in the cell membrane indicating that PS particles were possibly entering the bacterial cells. Transcriptome analyses performed to identify the differentially expressed genes upon PS exposure revealed that the genes involved in major virulence factor type VI secretion system (T6SS) were down-regulated. However, the expression of T6SS-related genes was recovered from the PS adapted E. piscicida when nanoplastics are free. This demonstrated the hypervirulence of pathogen in infection assays with both cell lines and in vivo zebrafish model. Therefore, this study provides experimental evidence elucidating the direct regulatory impact of nanoplastics influx into aquatic ecosystems on fish pathogenic bacteria, notably influencing the expression of virulence factors.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Edwardsiella / Poluentes Ambientais / Doenças dos Peixes Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Edwardsiella / Poluentes Ambientais / Doenças dos Peixes Idioma: En Ano de publicação: 2024 Tipo de documento: Article