Preparation of Soluble Mucin Solutions from the Salivary Glands.
Methods Mol Biol
; 2763: 45-50, 2024.
Article
em En
| MEDLINE
| ID: mdl-38347398
ABSTRACT
Studying salivary gland mucins is important for elucidating the pathogenesis of salivary gland diseases, including tumors and xerostomia, and developing diagnostic methods for them. Classic methods for isolating mucins from salivary glands require sacrificing several animals to obtain sufficient quantities of mucin and are time-consuming. Supported molecular matrix electrophoresis (SMME) was used to characterize mucins and their glycans. With this method, mucins can be analyzed within 2 days using less than 100 mg of tissue and without using expensive equipment, such as an ultracentrifuge. This chapter describes a method for preparing mucin solutions for SMME analysis of salivary gland mucins.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glândula Submandibular
/
Mucinas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article