Influence of adhesion-promoting glycolipids on the structure and stability of solid-supported lipid double-bilayers.
Soft Matter
; 20(9): 2113-2125, 2024 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-38349522
ABSTRACT
Glycolipids have a considerable influence on the interaction between adjacent biomembranes and can promote membrane adhesion trough favorable sugar-sugar "bonds" even at low glycolipid fractions. Here, in order to obtain structural insights into this phenomenon, we utilize neutron reflectometry in combination with a floating lipid bilayer architecture that brings two glycolipid-loaded lipid bilayers to close proximity. We find that selected glycolipids with di-, or oligosaccharide headgroups affect the inter-bilayer water layer thickness and appear to contribute to the stability of the double-bilayer architecture by promoting adhesion of adjacent bilayers even against induced electrostatic repulsion. However, we do not observe any redistribution of glycolipids that would maximize the density of sugar-sugar contacts. Our results point towards possible strategies for the investigation of interactions between cell surfaces involving specific protein-protein, lipid-lipid, or protein-lipid binding.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glicolipídeos
/
Bicamadas Lipídicas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article