Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus.
Stem Cell Res
; 76: 103332, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38354648
ABSTRACT
We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through real-time PCR and immunofluorescence, (OCT4, SOX2, TRA-1-60, NANOG and SSEA4) and in vitro differentiation into three germ layers.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes Induzidas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article