Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages.

Thygesen, Sara J; Burgener, Sabrina S; Mudai, Prerna; Monteleone, Mercedes; Boucher, Dave; Sagulenko, Vitaliya; Schroder, Kate; Stacey, Katryn J

Thygesen, Sara J; Burgener, Sabrina S; Mudai, Prerna; Monteleone, Mercedes; Boucher, Dave; Sagulenko, Vitaliya; Schroder, Kate; Stacey, Katryn J.

Afiliação

Thygesen SJ; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Australia.
Burgener SS; Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland, St Lucia, Australia.
Mudai P; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Australia.
Monteleone M; Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland, St Lucia, Australia.
Boucher D; York Biomedical Research Institute, Department of Biology, University of York, York, United Kingdom.
Sagulenko V; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Australia.
Schroder K; Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland, St Lucia, Australia.
Stacey KJ; Australian Infectious Diseases Research Centre, The University of Queensland, St Lucia, Australia.

Eur J Immunol ; 54(5): e2350515, 2024 May.

Article em En | MEDLINE | ID: mdl-38361219

ABSTRACT

ABSTRACT

Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.

Assuntos

Caspase 1; Inibidores de Caspase; Permeabilidade da Membrana Celular; Corantes Fluorescentes; Inflamassomos; Macrófagos; Caspase 1/metabolismo; Animais; Macrófagos/imunologia; Macrófagos/metabolismo; Permeabilidade da Membrana Celular/efeitos dos fármacos; Camundongos; Inflamassomos/metabolismo; Inibidores de Caspase/farmacologia; Camundongos Knockout; Proteínas de Ligação a Fosfato/metabolismo; Humanos

Palavras-chave

Caspase inhibitor; Caspase1; FLICA; Gasdermin D; Inflammasome

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Permeabilidade da Membrana Celular / Caspase 1 / Inflamassomos / Inibidores de Caspase / Corantes Fluorescentes / Macrófagos Idioma: En Ano de publicação: 2024 Tipo de documento: Article

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