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A Chromosome-Level Genome Assembly and Annotation for the Clouded Apollo Butterfly (Parnassius mnemosyne): A Species of Global Conservation Concern.
Höglund, Jacob; Dias, Guilherme; Olsen, Remi-André; Soares, André; Bunikis, Ignas; Talla, Venkat; Backström, Niclas.
Afiliação
  • Höglund J; Animal Ecology Program, Department of Ecology and Genetics (IEG), Uppsala University, Uppsala SE-752 36, Sweden.
  • Dias G; National Bioinformatics Infrastructure Sweden (NBIS), Science for Life Laboratory, Uppsala 752 37, Sweden.
  • Olsen RA; Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna 17165, Sweden.
  • Soares A; National Bioinformatics Infrastructure Sweden (NBIS), Science for Life Laboratory, Uppsala 752 37, Sweden.
  • Bunikis I; Uppsala Genome Center, Department of Immunology, Genetics and Pathology, Uppsala University, National Genomics Infrastructure hosted by SciLifeLab, Uppsala, Sweden.
  • Talla V; Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala 752 37, Sweden.
  • Backström N; Evolutionary Biology Program, Department of Ecology and Genetics (IEG), Uppsala University, Uppsala SE-752 36, Sweden.
Genome Biol Evol ; 16(2)2024 02 01.
Article em En | MEDLINE | ID: mdl-38368625
ABSTRACT
The clouded apollo (Parnassius mnemosyne) is a palearctic butterfly distributed over a large part of western Eurasia, but population declines and fragmentation have been observed in many parts of the range. The development of genomic tools can help to shed light on the genetic consequences of the decline and to make informed decisions about direct conservation actions. Here, we present a high-contiguity, chromosome-level genome assembly of a female clouded apollo butterfly and provide detailed annotations of genes and transposable elements. We find that the large genome (1.5 Gb) of the clouded apollo is extraordinarily repeat rich (73%). Despite that, the combination of sequencing techniques allowed us to assemble all chromosomes (nc = 29) to a high degree of completeness. The annotation resulted in a relatively high number of protein-coding genes (22,854) compared with other Lepidoptera, of which a large proportion (21,635) could be assigned functions based on homology with other species. A comparative analysis indicates that overall genome structure has been largely conserved, both within the genus and compared with the ancestral lepidopteran karyotype. The high-quality genome assembly and detailed annotation presented here will constitute an important tool for forthcoming efforts aimed at understanding the genetic consequences of fragmentation and decline, as well as for assessments of genetic diversity, population structure, inbreeding, and genetic load in the clouded apollo butterfly.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Borboletas Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Borboletas Idioma: En Ano de publicação: 2024 Tipo de documento: Article