Disruption of spermatogenesis in testicular adult Wistar rats after short-term exposure to high dose of glyphosate based-herbicide: Histopathological and biochemical changes.
Reprod Biol
; 24(2): 100865, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38402720
ABSTRACT
Glyphosate is an endocrine disruptor and can act on the activity of certain enzymes of metabolism subsequently altering some functions such as reproduction. The goal of the present study is to evaluate the involvement of glyphosate based-herbicide (GBH) in spermatogenesis disruption and to investigate which cells of the adult Wistar rat testis are most affected by short-term exposure to GBH. Treated groups received a diluted solution of GBH orally for 21 days (D1 102.5 mg/Kg; D2 200 mg/Kg; D3 400 mg/Kg). The control group (C) received water in the same manner. Hormone levels, oxidative stress markers were evaluated, histological and morphometric analysis were performed, AR and p53 expression was conducted. Seminiferious epithelium sloughing associated to erosion of Sertoli and spermatogonia from the basement of the seminiferous tubules, with intraluminal exfoliated cells among with immature spermatids were observed. A significant change in morphometric measurement and significant decrease in AR expression in Sertoli cells were noted for all treated groups. A significant increase in NO level and p53 expression in Leydig cells were showed for animals treated with 200 and 400 mg/kg BW/day. These data demonstrate that short-term exposure to high doses of GBH has led to a disruption of certain parameters that could disturb spermatogenesis. The treatment showed that both Leydig and Sertoli cells are affected in the same manner by GBH, the activation of p53 expression in both Leydig cells and peritubular myloid cells nuclei, and the reduction in AR expression in Sertoli cells, which resulted in important testicular damage.
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MEDLINE
Assunto principal:
Espermatogênese
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Testículo
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Ratos Wistar
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Glifosato
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Glicina
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Herbicidas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article