Multibeam continuous axial scanning two-photon microscopy for in vivo volumetric imaging in mouse brain.
Biomed Opt Express
; 15(2): 1089-1101, 2024 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-38404301
ABSTRACT
This study presents an alternative approach for two-photon volumetric imaging that combines multibeam lateral scanning with continuous axial scanning using a confocal spinning-disk scanner and an electrically focus tunable lens. Using this proposed system, the brain of a living mouse could be imaged at a penetration depth of over 450â
µm from the surface. In vivo volumetric Ca2+ imaging at a volume rate of 1.5â
Hz within a depth range of 130-200â
µm, was segmented with an axial pitch of approximately 5-µm and revealed spontaneous activity of neurons with their 3D positions. This study offers a practical microscope design equipped with compact scanners, a simple control system, and readily adjustable imaging parameters, which is crucial for the widespread adoption of two-photon volumetric imaging.
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MEDLINE
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En
Ano de publicação:
2024
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Article