African swine fever virus early protein pI73R suppresses the type-I IFN promoter activities.
Virus Res
; 343: 199342, 2024 05.
Article
em En
| MEDLINE
| ID: mdl-38408646
ABSTRACT
African swine fever virus is known to suppress type-I interferon (IFN) responses. The main objective of this study was to screen early-expressed viral genes for their ability to suppress IFN production. Out of 16 early genes examined, I73R exhibited robust suppression of cGAS-STING-induced IFN-ß promoter activities, impeding the function of both IRF3 and NF-κB transcription factors. As a result, I73R obstructed IRF3 nuclear translocation following the treatment of cells with poly(dAdT), a strong inducer of the cGAS-STING signaling pathway. Although the I73R protein exhibits structural homology with the Zα domain binding to the left-handed helical form of DNA known as Z-DNA, its ability to suppress cGAS-STING induction of IFN-ß was independent of Z-DNA binding activity. Instead, the α3 and ß1 domains of I73R played a significant role in suppressing cGAS-STING induction of IFN-ß. These findings offer insights into the protein's functions and support its role as a virulence factor.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Interferon Tipo I
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Febre Suína Africana
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Vírus da Febre Suína Africana
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DNA Forma Z
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article