Probing cerebellar circuit dysfunction in rodent models of spinocerebellar ataxia by means of in vivo two-photon calcium imaging.
STAR Protoc
; 5(1): 102911, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38412102
ABSTRACT
Purkinje neuron degeneration characterizes spinocerebellar ataxia type 1, yet the comprehension of the impact on the broader cerebellar circuit remains incomplete. We here detail simultaneous in vivo two-photon calcium imaging of diverse neuronal populations in the cerebellar cortex of Sca1 mice while they are navigating a virtual environment. We outline surgical procedures and protocols to chronically record from identical neurons, and we detail data post-processing and analysis to delineate disease-related alterations in neuronal activity and sensorimotor-driven response properties. For complete details on the use and execution of this protocol, please refer to Pilotto et al.1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Ataxias Espinocerebelares
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article