Potential inhibitory effect of Auricularia auricula polysaccharide on advanced glycation end-products (AGEs).
Int J Biol Macromol
; 262(Pt 1): 129856, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38423908
ABSTRACT
In this study, a novel polysaccharide, AAP-2S, was extracted from Auricularia auricula, and the anti-glycosylation effect of AAP-2S and its underlying mechanisms were investigated using an in vitro BSA-fructose model and a cellular model. The results demonstrated the inhibiting formation of advanced glycation end products (AGEs) in vitro by AAP-2S. Concurrently, it attenuated oxidative damage to proteins in the model, preserved protein sulfhydryl groups from oxidation, reduced protein carbonylation, prevented structural alterations in proteins, and decreased the formation of ß-crosslinked structures. Furthermore, AAP-2S demonstrated metal-chelating capabilities. GC-MS/MS-based metabolomics were employed to analyze changes in metabolic profiles induced by AAP-2S in a CML-induced HK-2 cell model. Mechanistic investigations revealed that AAP-2S could mitigate glycosylation and ameliorate cell fibrosis by modulating the RAGE/TGF-ß/NOX4 pathway. This study provides a foundational framework for further exploration of Auricularia auricular polysaccharide as a natural anti-AGEs agent, paving the way for its potential development and application as a food additive.
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Base de dados:
MEDLINE
Assunto principal:
Reação de Maillard
/
Auricularia
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article