Structural determination and modeling of ciliary microtubules.
Acta Crystallogr D Struct Biol
; 80(Pt 4): 220-231, 2024 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-38451206
ABSTRACT
The axoneme, a microtubule-based array at the center of every cilium, has been the subject of structural investigations for decades, but only recent advances in cryo-EM and cryo-ET have allowed a molecular-level interpretation of the entire complex to be achieved. The unique properties of the nine doublet microtubules and central pair of singlet microtubules that form the axoneme, including the highly decorated tubulin lattice and the docking of massive axonemal complexes, provide opportunities and challenges for sample preparation, 3D reconstruction and atomic modeling. Here, the approaches used for cryo-EM and cryo-ET of axonemes are reviewed, while highlighting the unique opportunities provided by the latest generation of AI-guided tools that are transforming structural biology.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Axonema
/
Microtúbulos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article