The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes.
Virol Sin
; 39(2): 228-234, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38461965
ABSTRACT
Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from Culex spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 âcells. Further in vitro characterization revealed that the two GCXVs exhibited comparable replication kinetics, protein expression and viral titers. Importantly, GCXV RNAs were detected in the bodies, salivary glands, midguts and ovaries of Culex quinquefasciatus at 4-10 days after oral infection. In addition, two GCXVs can colonize Cx. quinquefasciatus eggs, resulting in positive rates of 15%-35% for the second gonotrophic cycle. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in Cx. quinquefasciatus eggs during the first and second gonotrophic cycles after oral infection.
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Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
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RNA Viral
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Culex
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Mosquitos Vetores
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article