[Effects of Combined Stress of High Density Polyethylene Microplastics and Chlorimuron-ethyl on Soybean Growth and Rhizosphere Bacterial Community].
Huan Jing Ke Xue
; 45(2): 1161-1172, 2024 Feb 08.
Article
em Zh
| MEDLINE
| ID: mdl-38471953
ABSTRACT
With the vigorous development of agriculture in Chinaï¼ plastic mulch film and pesticides are widely used in agricultural production. Howeverï¼ the accumulation of microplastics ï¼formed by the degradation of plastic mulch filmï¼ and pesticides in soil has also caused many environmental problems. At presentï¼ the environmental biological effects of microplastics or pesticides have been reportedï¼ but there are few studies on the combined effects on crop growth and the rhizosphere soil bacterial community. Thereforeï¼ in this studyï¼ the high density polyethylene microplastics ï¼HDPEï¼ 500 meshï¼ were designed to be co-treated with sulfonylurea herbicide chlorimuron-ethyl to study their effects on soybean growth. In additionï¼ the effects of the combined stress of HDPE and chlorimuron-ethyl on soybean rhizosphere soil bacterial community diversityï¼ structure compositionï¼ microbial community networkï¼ and soil function were investigated using high-throughput sequencing technologyï¼ interaction networkï¼ and PICRUSt2 function analysis to clarify the combined toxicity of HDPE and chlorimuron-ethyl to soybean. The results showed that the half-life of chlorimuron-ethyl in soil was prolonged by the 1% HDPE treatment ï¼from 11.5 d to 14.3 dï¼ï¼ and the combined stress of HDPE and chlorimuron-ethyl had more obvious inhibition effects on soybean growth than that of the single pollutant or control. The HiSeq 2 500 sequencing showed that the rhizosphere bacterial community of soybean was composed of 20 phyla and 312 genera under combined stressï¼ the number of phyla and genera was significantly less than that of the control and single pollutant treatmentï¼ and the relative abundances of bacteria with potential biological control and plant growth-promoting characteristics ï¼such as Nocardioides and Sphingomonasï¼ were reduced. Alpha diversity analysis showed that the combined stress significantly reduced the richness and diversity of the soybean rhizosphere bacterial communityï¼ and Beta diversity analysis showed that the combined stress significantly changed the structure of the bacterial community. The dominant flora of the rhizosphere bacterial community were regulatedï¼ and the abundances of secondary functional layers such as amino acid metabolismï¼ energy metabolismï¼ and lipid metabolism were reduced under combined stress by the analysis of LEfSe and PICRUSt2. It was inferred from the network analysis that the combined stress of HDPE and chlorimuron-ethyl reduced the total number of connections and network density of soil bacteriaï¼ simplified the network structureï¼ and changed the important flora species to maintain the stability of the network. The results above indicated that the combined stress of HDPE and chlorimuron-ethyl significantly affected the growth of soybean and changed the rhizosphere bacterial community structureï¼ soil functionï¼ and network structure. Compared with that of the single pollutant treatmentï¼ the potential risk of combined stress was greater. The results of this study can provide guidance for evaluating the ecological risks of polyethylene microplastics and chlorimuron-ethyl and for the remediation of contaminated soil.
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Base de dados:
MEDLINE
Assunto principal:
Pirimidinas
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Compostos de Sulfonilureia
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Poluentes Ambientais
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Herbicidas
Idioma:
Zh
Ano de publicação:
2024
Tipo de documento:
Article